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Whole Genome Bisulfite Sequencing

Whole Genome Bisulfite Sequencing (WGBS)

Whole Genome Bisulfite Sequencing (WGBS) provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Tell us about your project, our experts are here to help.

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Whole Genome Bisulfite Sequencing Introduction

Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.

Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.

Cancer: Breast cancer, Bladder cancer, Lymph cancer Liver cancer

Complex Diseases: Autoimmune, Cardiovascular, Mental Disorders, Genetic Imprinting

Other Apps: Cell Differentiation, Embryonic Development, Methylation Mapping

Whole Genome Bisulfite Sequencing (WGBS)-BGI Service Overview

Sample Requirements

 Mass:≥0.5 μg

 Concentration:≥12.5 ng/μL

 Volume:15 μL

Sequencing Standards

 Guaranteed ≥90% of clean bases with quality score of Q20

 Guaranteed ≥99% bisulfite conversion rate

 Standard sequencing coverage ≥30X is recommended

Key Service Details

 Typical 26 working days from sample QC acceptance to filtered raw data availability

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Whole Genome Bisulfite Sequencing Technology

BGI Genomics' Whole Genome Bisulfite Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.

Contact us with any general enquiries or questions for our sequencing experts, we are here to help.

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Access Further Sequencing Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below for full access to our range of educational resources and sample guides.

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