Service Description
BGI Genomics has extensive experience in the field of targeted metabolomics spanning accurate quantification of target metabolites, verification of hypothesis by untargeted metabolomics or other omics, and metabolic model study for specific metabolites1.
HM Pro2300
The HM Pro2300 panel uses LC-MS/MS technology for high-throughput targeted metabolomics detection. HM Pro2300 can simultaneously detect more than 2000 metabolites, including 700 small molecules and more than 1600 lipids which covers all kinds of important metabolites and core metabolic pathways. We also provide HM700 and HML1600, only detecting hydrophilic small molecules and lipids respectively.
Project Workflow
References
[1] Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.
How to order
Mass Spectrometry Service Specification
Sample Preparation and Services
- Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology
- Small molecules and lipids are extracted respectively to increase metabolome depth
- Using SCIEX QTRAP 6500+, 5500 detect respectively small molecules and lipids to increase the scope of detection
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format
Turn Around Time
- Typical 6-8 weeks after sample receipt
Sample Requirements
| SAMPLE TYPE | RECOMMENDED SAMPLE AMOUNT | MINIMUM SAMPLE AMOUNT |
|---|---|---|
| Serum, plasma, urine | ≥ 200 µL | ≥ 100 µL |
| Animal and clinical tissues, feces and intestinal contents, microorganism | ≥ 100 mg | ≥ 40 mg |
| Cell | 2×10^7 | 2×10^5 |
| Culture medium, fermentation medium | ≥ 1 mL | ≥ 200 µL |
Data Analysis
- Data analysis performed with metaX and BGI Genomics library
- Data quality control
- Standard curves
- Quantification results
