Service Description
BGI Genomics has extensive experience in the field of targeted metabolomics spanning accurate quantification of target metabolites, verification of hypothesis by untargeted metabolomics or other omics, and metabolic model study for specific metabolites1.
HM700
The HM700 panel uses LC-MS/MS technology for high-throughput targeted metabolomics detection. It covers around 700 metabolites including 30 metabolite classes addressing key pathophysiological processes relevant in gut microbiota research and cancer research areas.
This enables greater in-depth mining of metabolome information, while providing accurate quantification, excellent reproducibility, and high throughput. Each metabolite is quantitatively determined using the standard curve.
Project Workflow
References
[1] Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.
How to order
Mass Spectrometry Service Specification
Sample Preparation and Services
- Each metabolite has a corresponding two kinds of standard curves with broad dynamic range for absolute quantification and was corrected using isotope internal standard
- 3-NPH derivatization significantly achieve ultra-high
sensitivity and cross-magnitude detection
- Using LC-MS (SCIEX QTRAP 6500+) to increase the scope of detection
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format
Turn Around Time
- Typical 5-7 weeks after sample receipt
Sample Requirements
| SAMPLE TYPE | RECOMMENDED SAMPLE AMOUNT | MINIMUM SAMPLE AMOUNT |
|---|---|---|
| Serum, plasma, urine | ≥ 100 µL | ≥ 50 µL |
| Animal and clinical tissues, feces and intestinal contents, microorganism | ≥ 50 mg | ≥ 20 mg |
| Cell | 1×10^7 | 1×10^5 |
| Culture medium, fermentation medium | ≥ 500 µL | ≥ 100 µL |
Data Analysis
- Data analysis performed with metaX and BGI Genomics library
- Data quality control
- Standard curves
- Quantification results
