DESCRIPTION
SPECIFICATION
RESOURCES
Project Workflow
- PROTEIN EXTRACTION
- PROTEIN DIGESTION
- HPLC FRACTIONATION
- LC-MS/MS (DDA)
- DATA ANALYSIS
How to order
Mass Spectrometry Service Specification
Label-free DDA Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Triple TOF 5600).
Sample Preparation and Services
- The QC of enzymatic hydrolysis is strictly controlled by LC-MS
- 2h for DDA analysis
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 3-4 weeks from sample QC acceptance to data report delivery
Sample Requirements
| SAMPLE TYPE | AMOUNT AND CONCENTRATION | MINIMUM SAMPLE VOLUME |
|---|---|---|
| Protein sample in liquid solution | Recommended 400 µg with fractionation: 2 µg/µL | 200 µL |
| Protein sample in liquid solution | Minimum Required 100 µg with fractionation: 1 µg/µL | 100 µL |
Data Analysis
DATA ANALYSIS
- Data analysis and validation performed with Mascot
- Protein identification and quantification list
- Differential proteins data statistics and volcano plot
- Principal component analysis (PCA) and cluster analysis
- Protein GO/COG/KOG/Pathway annotation
- GO/COG/KOG enrichment analysis of differential proteins
- Protein-protein interaction analysis
- Protein subcellular localization analysis
- Time series analysis
- Repeatability analysis
Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
