DESCRIPTION
SPECIFICATION
RESOURCES
Project Workflow
DDA analysis can be carried out if there are no suitable library construction samples:
- Protein extraction
- Protein digestion
- Individual samples
- LC-MS/MS (DDA)
DIA analysis can be carried out if there are suitable library construction samples:
References
[1] Kleiner M. Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities. mSystems. 2019 May-Jun; 4(3): e00115-19.
How to order
Mass Spectrometry Service Specification
Metaproteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Orbitrap Fusion Lumos).
Sample Preparation and Services
- The QC analysis of enzymatic hydrolysis is strictly controlled by LC-MS
- DDA-Spectral Library generated using HPLC technology and sample fractionation
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 4-5 weeks from sample QC acceptance to data report delivery
Data Analysis
DATA ANALYSIS
- Data analysis and validation performed with Mascot
- Protein Analysis
- Species analysis
- Diversity analysis
- Function Analysis
- Correlation Analysis
