Description
Specification
Resources
Project Workflow
References
[1] L. Yi, et al., Advances in microscale separations towards nanoproteomics applications, 2017.1523: 40–48.
How to order
Mass Spectrometry Service Specification
Nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge Orbitrap Fusion Lumos mass spectrometer.
Sample Preparation and Services
- The QC of enzymatic hydrolysis is strictly controlled by LC-MS
- DDA-Spectral Library generated using HPLC technology and sample fractionation
- 2h/fraction; 2h for DDA analysis
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 5-6 weeks from sample QC acceptance to data report delivery
Sample Requirements
DATA ANALYSIS
- Data analysis and validation performed with Mascot
- Protein identification and quantification list
- Cluster analysis of expression patterns across multiple samples
- Protein GO/COG/KOG/Pathway annotation
- GO/Pathway enrichment analysis of differential proteins
- Protein-protein interaction analysis
- Protein subcellular localization analysis
- Repeatability analysis
- Quantitative proteomics and phosphoproteomics correlation analysis
