Service Description
Metabolome + Metagenome / 16S Analysis
In recent years, research on gut related diseases has developed rapidly and it is now thought that nearly 90% of diseases may be related to gut microbiota health. Metagenome/16S + metabolome correlation analysis enables researchers to establish a correlation model between host metabolism and gut microbiota and explore the causal relationships between microbes and disease1-6.
Project Workflow
Note: Stools and intestinal contents samples are recommended for Metagenome/16S services; blood samples (serum, plasma) are recommended for Metabolome services. No biological correlation analysis for the differential metabolites and the differential species.
References
[1] Mendelian randomization analyses support causal relationships between blood metabolites and the gut microbiome. Nature Genetics. 2022 Jan; 54(1): 52-61. (BGI's Publication)
[2] Endosymbionts reduce microbiome diversity and modify host metabolism and fecundity in the planthopper Sogatella furcifera. mSystems. 2022 Apr 26; 7(2): e0151621. (BGI's Customer)
[3] Life history recorded in the vagino-cervical microbiome along with multi-omics. Genomics Proteomics Bioinformatics. 2021 Jun 9: S1672-0229(21)00099-1. (BGI's Publication)
[4] Metagenome-wide association of gut microbiome features for schizophrenia. Nat Commun. 2020 Mar 31;11(1):1612. (BGI's Publication)
[5] Transplantation of microbiota from drug-free patients with schizophrenia causes schizophrenia-like abnormal behaviors and dysregulated kynurenine metabolism in mice. Mol Psychiatry. 2020 Nov;25(11):2905-2918. (BGI's Publication)
[6] Gut microbiome-related effects of berberine and probiotics on type 2 diabetes. Nat Commun. 2020 Oct 6;11(1):5015. (BGI's Publication)
How to order
Mass Spectrometry Service Specification
Quality Standard
- Summary includes data analysis
- Reports provided in PDF format, RAW files available upon request
Turn Around Time
- Typical 2 weeks from LC-MS/MS raw data acceptance to data report delivery
Sample Requirements
| INTEGRATIVE ANALYSIS | BIOLOGICAL DUPLICATES WHEN 2 GROUPS | BIOLOGICAL DUPLICATES WHEN ≥ 3 GROUPS |
|---|---|---|
| Metagenome + metabolome | ≥ 8 | ≥ 6 |
| 16S+ metabolome | ≥ 8 | ≥ 4 |
| Clinical samples | ≥ 30 | ≥ 30 |
Data Analysis
- Univariate correlation
- Unsupervised model correlation
- Supervised model correlation
- Biological function correlation
